phenol red free dmem f12 medium (Thermo Fisher)

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Thermo Fisher phenol red free dmem f12 medium
Phenol Red Free Dmem F12 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+dmem+f12/Phenol+Red/pm42274549-50-7-24
Average 99 stars, based on 1 article reviews

phenol red free dmem f12 medium - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Preclinical study of red dragon fruit ( <i>Hylocereus polyrhizus</i> ) betacyanins in the G93A mutant hSOD1 mouse model of amyotrophic lateral sclerosis
Article Snippet: Differentiation media was switched every other day for 96 h. After differentiation, cells were swapped to a post-differentiation media containing phenol red-free DMEM/F12, 1% insulin transferrin selenium supplement (Gibco 41400045), 1% non-essential amino acids, and 1% pen/strep for experimentation, as previously described [22].

Article Title: PBK-Loaded secretory autophagosomes drive radiotherapy-induced systemic adipose depletion via MAPK/ERK-PRKA/PKA-LIPE/HSL signaling: a therapeutic target for esophageal cancer cachexia.
Article Snippet: Radiotherapy, while a cornerstone treatment for esophageal squamous cell carcinoma (ESCC), is paradoxically associated with significant weight loss that portends poor patient outcomes.. The mechanisms driving this metabolic complication remain elusive.. Here, we identified adipose depletion—rather than muscle atrophy—as the primary contributor to radiotherapy-induced weight loss in ESCC.

Article Title: A one-step, resin-compatible Cys-Trp cross-linking system opens a new avenue for stapled peptide therapeutics.
Article Snippet: Protein-protein interactions (PPIs) are attractive yet challenging drug targets, motivating the development of conformationally constrained peptides such as stapled α-helices.. Here we report a one-pot cysteine-tryptophan (Cys-Trp) cross-linking strategy that enables direct formation of Trp-indole C–H sulfenylation staples concomitant with global side-chain deprotection and resin cleavage.. Application of this approach afforded a CysTrp-stapled analogue of estrogen receptor-α (ERα) activity regulator synthetic peptide (ERAP), designed to disrupt a pathogenic PPI implicated in breast cancer.

Article Title: LIPUS promotes stem leydig cells proliferation by up-regulating Wnt5a expression and delays male aging.
Article Snippet: The induction medium consisted of phenol red-free DMEM/F12 (21041025, Gibco) supplemented with 2% fetal bovine serum (A5256701, Gibco), 1 ng/mL luteinizing hormone (LH) (869003-M, Sigma), 1 nM triiodothyronine (T3) (T2877, Sigma),, 10 ng/mL PDGF-BB, 70 ng/mL insulin-like growth factor 1 (IGF-1) (C032, Novoprotein), and a 1× ITS supplement.

Irradiation:

Article Title: The atypical E3 ligase HOIL-1 safeguards the ribosome during cellular stress
Article Snippet: UV-C irradiation was performed using a UV crosslinker (Analytik Jena CL-1000) set to 254 nm for the indicated doses. .. Then, 1 h before irradiation, the medium was aspirated and cells were washed twice with pre-warmed PBS and replaced with phenol red-free DMEM/F12 (Gibco, 21041025) containing 10% FBS. ..

Cell Culture:

Article Title: NR5A2 increases inflammation and cell proliferation by regulating the nuclear factor κB pathway in polyendocrine metabolic ovarian syndrome
Article Snippet: This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo editing, typesetting, and review of the resulting proof before it is published in its final form.

Incubation:

Article Title: High Uric Acid Promotes Stem Leydig Cell Senescence by CCDC90B Mediates Mitochondrial Quality Control Imbalance.
Article Snippet: .. The medium consisted of phenol red- free DMEM/F12 and M199 medium (1:1; Gibco) supplemented with 2% FBS, 10 ng/mL PDGFAA (PeproTech), 1 ng/mL LH (R&D Systems), 250 nM Smoothened Agonist HCl (SAG; Millipore), 1 μM Forskolin (Fsk; Sigma- Aldrich), 50 ng/ mL insulin- like growth factor 1 (IGF1, PeproTech) and 5 μg/L insulin- transferrin- sodium selenite (Sigma- Aldrich) and they were incubated for 14 days. ..

Imaging:

Article Title: Electroporation-mediated delivery of protein biosensors for metabolic imaging in differentiated myotubes
Article Snippet: .. After 24 h or 6–11 days depending on the experiment, the medium was replaced with phenol red-free DMEM/F12 (# 11039-021, Thermo Fisher Scientific) for imaging. .. For suspended cells, electroporation was performed using a CUY21EDITII electroporator (BEX Co., Ltd, Tokyo, Japan) following the manufacturer’s protocol.

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